Honeysuckle-derived microRNA2911 inhibits tumor growth by targeting TGF-β1

Abstract Background Honeysuckle is a time‐honored herb with anticancer activity in traditional Chinese medicine. Recently, accumulating reports are suggesting that the microRNAs this medicinal plant not only play physiological role their original system, but also can be transmitted to another species as potential therapeutic components. In numerous bioactive investigations, anti-tumor effects of these magical rarely studied, especially special miR2911, honeysuckle-encoded atypical microRNA, high stability during boiling process and unique biological target TGF-β1 mRNA. Methods Luciferase assay was conducted test ability miR2911 ELISA performed determine expression level mouse colorectal adenocarcinoma CT26 cells when treated tumor tissue Sidt1 + / −/− mice. qRT-PCR examine miR2911. Tumor-bearing wild nude mice were employed evaluate effect honeysuckle vivo. Tumor necrosis observed by H&E staining. Besides, infiltration T lymphocytes across solid tumors tested immunostaining Results Our results showed slowed development colon cancer down. Further research could bind strongly mRNA down-regulate had under acid condition. Moreover, SIDT1 mediated dietary inter-species absorption. And we found similar honeysuckle. Mechanistically, reversed tumor-promoting an increase infiltration, resulting slowing immunocompetent Consistent inference, revealed abolished cell immune deficiency Conclusion Taken together, honeysuckle-derived through targeting The down-regulation promoted accordingly impeded development.